The European Commission’s revised Market Definition Notice, published in 2024, brought many changes and updates to this central analytical step in EU competition law. A Special Issue in the Journal of Antitrust Enforcement, published in March 2026, puts a spotlight on the Notice’s novelties, its intricacies and the questions it raises. Covering issues as diverse as labour market definition, systemic market power, ecosystems, intellectual property rights, homogeneous conditions of competition and multi-sided markets, the Special Issue contains contributions by Terézia Ovečka, Joanna Mazur and Antoni Napieralski, Emanuela Lecchi, Annika Stöhr and Oliver Budzinski, Anouk van der Veer, Hedvig Schmidt, Lirio Barros, Timo Klein, Sophie Kümmel, and Ilaria Noviello, Jan Broulík, as well as Helen Ralston-Smith and Gunnar Niels. The Special Issue was brought together by guest editors Magali Eben (CREATe, University of Glasgow) and The Hub’s Vicky Robertson (WU Vienna). Their editorial can be read in open access here.